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ATCC
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ATCC
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Image Search Results
Journal: medRxiv
Article Title: Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity
doi: 10.1101/2021.09.01.21262715
Figure Lengend Snippet: a Number of patients recruited in the DRASTIC cohort and samples collected (top panel) and days post disease onset of COVID-19 blood samples (bottom panel). b Collection timepoints of respiratory samples (endotracheal tube aspirate (ETA), sputum, and pleural fluid) and paired blood samples from the same patients (left panel). Comparison of days post disease onset between blood and respiratory samples for COVID-19 and non-COVID-19 patients (right panel) using Mann-Whitney test. c Maximum likelihood phylogenetic tree of SARS-CoV-2 sequences from Victoria from 28 January 2020 to 28 October 2020 (including context sequences from rest of Australia and New Zealand). Phylogenetic tree includes randomly subsampled sequences from transmission networks (TN) A and TN B in Victoria, with a total number of 10941 and 145 cases respectively. The outermost tip of each radial line represents a single sequence; the sum of each radial line between two tips represents the genetic distance between two sequences. Each radial stepwise progression represents approximately one single nucleotide polymorphism (SNP). Sequences from study patients are shown as open circles (patient with blood samples only) or solid-coloured circles (patients with blood and respiratory samples, same colours as in section b). Half-filled circles are used when samples are located close to each other. d Distribution of clinical data in ward and intensive care unit (ICU) COVID-19 patients. Box and bars indicate first and third quartiles, and range respectively. Statistical significance was determined with the Fisher’s exact test for the National Institutes of Health (NIH) score, and Mann-Whitney test for age, weight, height, and body weight index (BMI). Correlation was determined with Spearman’s correlation.
Article Snippet: In addition,
Techniques: MANN-WHITNEY, Transmission Assay, Sequencing
Journal: medRxiv
Article Title: Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity
doi: 10.1101/2021.09.01.21262715
Figure Lengend Snippet: a ELISA titration curves against the SARS-CoV-2 receptor-binding domain (RBD) for IgM, IgG, and IgA in COVID-19 respiratory and paired blood samples and non-COVID-19 respiratory samples. Dotted lines within each graph indicates the cut-off used to determine end-point titres. b End-point titres of SARS-CoV-2 RBD antibodies between (i) respiratory samples of COVID-19 and non-COVID-19 patients, and (ii) plasma and respiratory samples of COVID-19 patients. (i) Bars indicate median with interquartile range. Dotted line indicates the detection level. (ii) Dotted lines connect the most closely matched plasma and respiratory samples from each patient. Statistical significance was determined with Mann-Whitney test. c ELISA titration curves against the SARS-CoV-2 RBD for 3 COVID-19 patients with serial respiratory samples. d Heatmap of percentage (%) inhibition tested by surrogate virus neutralization test (sVNT) and anti-RBD ELISA titres. e Correlation between anti-RBD antibody titres and (%) sVNT inhibition. Correlation was determined with Spearman’s correlation. f Number of (i) samples and (ii) patients with seroconverted anti-RBD IgM, IgG, IgA and positive % sVNT inhibition. Pink curved lines surrounding the donut graphs indicate the samples/patients with seroconverted IgM. Earliest samples were used for each patient when determining seroconversion which was defined as average titre +2×SD of non-COVID-19 samples. Positive % sVNT inhibition was defined as % sVNT inhibition ≥ 20%.
Article Snippet: In addition,
Techniques: Enzyme-linked Immunosorbent Assay, Titration, Binding Assay, MANN-WHITNEY, Inhibition, Neutralization
Journal: medRxiv
Article Title: Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity
doi: 10.1101/2021.09.01.21262715
Figure Lengend Snippet: a Heatmaps with unsupervised clustering of SARS-CoV-2-specific antibodies in COVID-19 respiratory and plasma samples. b median fluorescence intensity of IgM, IgG, IgA1, and IgA2 antibodies between COVID-19 and non-COVID-19 respiratory samples. Statistical significance was determined with Mann-Whitney test. c Partial Least-Squares Discriminant Analysis (PLSDA) scores and loading plots of ETA and plasma from five COVID-19 and five non-COVID-19 patients with the smallest difference in days post disease onset between ETA and plasma samples.
Article Snippet: In addition,
Techniques: Fluorescence, MANN-WHITNEY